High-temperature optima phosphatases from the cold-tolerant Arctic fungus Penicillium citrinum

  • Puja Gawas-Sakhalkar
  • Shiv Mohan Singh National Centre of Antarctic & Ocean Research, Ministry of Earth Sciences,
  • Naik Simantini
  • Rasik Ravindra
Keywords: acid phosphatase, enzyme, para-nitrophenylphosphate, Pikovskaya medium, psychrotolerant, SDSPAGE

Abstract

Fifty-six fungal isolates from Arctic soils were subjected to primary screening for their ability to solubilize insoluble inorganic phosphate. Nine of the isolates were further analysed quantitatively for phosphatase production using para-nitrophenylphosphate as substrate. Amongst these, a cold-tolerant fungus, Penicillium citrinum strain PG162 was found to be the best producer of intracellular acid phosphatase. Further characterization of the enzyme showed that it is most active in the temperature range of 40-60°C and pH range of 4.2-4.8. The dried enzyme extract is stable at a temperature of up to 50°C for at least 1 h. Its activity is affected by presence of metal ions. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS–PAGE) analysis suggests the molecular weight of the enzyme to be between 20 and 29 kDa. The present study is important with respect to our understanding of the kind of enzymatic reactions that take place in the polar microbes, and the extent to which their activity is sustained.

Keywords: acid phosphatase; enzyme; para-nitrophenylphosphate; Pikovskaya medium; psychrotolerant; SDSPAGE

(Published: 9 August 2012)

Citation: Polar Research 2012, 31, 11105, http://dx.doi.org/10.3402/polar.v31i0.11105

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Author Biography

Shiv Mohan Singh, National Centre of Antarctic & Ocean Research, Ministry of Earth Sciences,

Scientist D, Polar Biology laboratory

Published
2012-08-09
How to Cite
Gawas-Sakhalkar, P., Singh, S., Simantini, N., & Ravindra, R. (2012). High-temperature optima phosphatases from the cold-tolerant Arctic fungus <em>Penicillium citrinum</em&gt;. Polar Research, 31. https://doi.org/10.3402/polar.v31i0.11105
Section
Research/review articles

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