Culturable heterotrophic bacteria from Potter Cove, Antarctica, and their hydrolytic enzymes production

  • Mauro Tropeano
  • Silvia Coria
  • Adrían Turjanski
  • Daniel Cicero
  • Andrés Bercovich
  • Walter Mac Cormack
  • Susana Vázquez Cátedra de Microbiología Industrial y Biotecnología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires CONICET
Keywords: Microbial enzymes, Antarctic bacteria, marine bacteria, cold enzymes, psychrophiles

Abstract

Affiliations of the dominant culturable bacteria isolated from Potter Cove, South Shetland Islands, Antarctica, were investigated together with their production of cold-active hydrolytic enzymes. A total of 189 aerobic heterotrophic bacterial isolates were obtained at 4°C and sorted into 63 phylotypes based on their amplified ribosomal DNA restriction analysis profiles. The sequencing of the 16S rRNA genes of representatives from each phylotype showed that the isolates belong to the phyla Proteobacteria (classes Alpha- and Gamma-proteobacteria), Bacteroidetes (class Flavobacteria), Actinobacteria (class Actinobacteria) and Firmicutes (class Bacilli). The predominant culturable group in the site studied belongs to the class Gammaproteobacteria, with 65 isolates affiliated to the genus Pseudoalteromonas and 58 to Psychrobacter. Among the 189 isolates screened, producers of amylases (9.5%), pectinases (22.8%), cellulases (14.8%), CM-cellulases (25.4%), xylanases (20.1%) and proteases (44.4%) were detected. More than 25% of the isolates produced at least one extracellular enzyme, with some of them producing up to six of the tested extracellular enzymatic activities. These results suggest that a high culturable bacterial diversity is present in Potter Cove and that this place represents a promising source of biomolecules.

Keywords: Microbial enzymes; Antarctic bacteria; marine bacteria; cold enzymes; psychrophiles

(Published: 20 December 2012)

Citation: Polar Research 2012, 31, 18507, http://dx.doi.org/10.3402/polar.v31i0.18507

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Published
2012-12-20
How to Cite
Tropeano, M., Coria, S., Turjanski, A., Cicero, D., Bercovich, A., Mac Cormack, W., & Vázquez, S. (2012). Culturable heterotrophic bacteria from Potter Cove, Antarctica, and their hydrolytic enzymes production. Polar Research, 31. https://doi.org/10.3402/polar.v31i0.18507
Section
Research/review articles